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KMID : 0613820190290111273
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Journal of Life Science
2019 Volume.29 No. 11 p.1273 ~ p.1280
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Ethanol Extract of Glycyrrhiza uralensis Protects Against Oxidative Stress-induced DNA Damage and Apoptosis in Retinal Pigment Epithelial Cells
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Kim So-Young
Kim Jeong-Hwan
Kim Sung-Ok
Park Seh-Kwang
Jeong Ji-Won
Kim Mi-Young
Lee Hye-Sook
Cheong Jae-Hun
Choi Yung-Hyun
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Abstract
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Age-related macular degeneration (AMD) is one of the leading causes of blindness in the elderly population, and damage to retinal pigment epithelial (RPE) cells due to oxidative stress contributes to the development of AMD. Glycyrrhiza uralensis Fischer is one of the most widely used herbal medicines for the treatment of various diseases in Asian countries. Although recent studies indicated that treatment with G. uralensis can protect cells from oxidative stress, its mechanisms in RPE cells remain unknown. We evaluated the effect of a G. uralensis ethanol extract (GU) on H2O2-induced oxidative injury in ARPE-19 RPE cells. The GU pretreatment attenuated reactive oxygen species (ROS) generation induced by H2O2, which was associated with induced expression of nuclear factor erythroid-derived-2-like 2 (Nrf2) and heme oxygenase-1 (HO-1). GU also suppressed H2O2-induced DNA damage and mitochondrial dysfunction. The inhibitory effect of GU on H2O2-induced apoptosis was associated with the protection of caspase-3 activation. Overall, GU appeared to protect RPE cells from oxidative injury by inhibiting DNA damage and reducing apoptosis. Further studies are needed to determine the regulation of Nrf2-mediated HO-1 expression, but our results suggest the possibility of using GU to reduce the risk of AMD.
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KEYWORD
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Apoptosis, DNA damage, Nrf2/HO-1, oxidative stress, retinal pigment epithelial cells
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